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ELISA kit for measuring Guinea Pig Urinary Protein allergen, GPUP. | ||||
Antibodies: | pAb/pAb | |||
Sensitivity: | 0.5ng/ml | |||
Limit of Detection: | 7.8ng/ml | |||
Assay Performance: | Inquire | |||
Contents: | Rabbit polyclonal antiserum, nGPUP Standard, Rabbit polyclonal antiserum; sufficient reagents for 10 microtiter plates. | |||
Storage: | Store at 4°C | |||
Not Provided: | 50mM carbonate/bicarbonate buffer, pH 9.6 | |||
Notes: | ||||
Product Resources: | EL-GPUP Certificate of Analysis | |||
For research and commercial use in vitro: not for human in vivo or therapeutic use. | ||||
Certificate of Analysis | ||||
Monoclonal Antibody: | Rabbit polyclonal antiserum | |||
Immunogen: | Guinea Pig Urinary Protein | |||
Isotype: | Multiple | |||
Specificity: | Binds to guinea pig urinary proteins. Reacts with guinea pig epithelial and hair extracts. Does not recognize rat or mouse urinary proteins; does not react with rabbit or hamster epithelial and hair extracts. | |||
Purification: | Affinity chromatography using recombinant Protein G. | |||
Concentration: | 1-2mg/ml in phosphate buffered saline, pH 7.4 Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. | |||
Certificate of Analysis | ||||
Antibody: | Rabbit polyclonal antiserum | |||
Specificity: | Binds to guinea pig urinary proteins. Reacts with guinea pig epithelial and hair extracts. Does not recognize rat or mouse urinary proteins; does not react with rabbit or hamster epithelial and hair extracts. | |||
Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1,000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4. 0.22μm filtered, preservative free. | |||
Certificate of Analysis | ||||
Allergen Standard: | nGPUP | |||
Composition: | Purified natural guinea pig urinary protein prepared in 1% BSA/50% glycerol/PBS, pH 7.4. | |||
Concentration: | 10,000ng/mL as determined by Advanced Protein Assay | |||
References: | ||||
1. Hilger C, Swiontek K, Kler S, Diederich C, Lehners C, Vogel L, Vieths S, Hentges F. Evaluation of two new recombinant guinea-pig lipocalins, Cav p 2 and Cav p 3, in the diagnosis of guinea-pig allergy. Clin Exp Allergy. 2011 Jun; 41(6):899-908. | ||||
2. Ferraz E, Arruda LK, Bagatin E, Martinez EZ, Cetlin AA, Simoneti CS, Freitas AS, Martinez JA, Borges MC, Vianna EO. Laboratory animals and respiratory allergies: the prevalence of allergies among laboratory animal workers and the need for prophylaxis. Clinics (Sao Paulo). 2013 Jun; 68(6): 750–759. | ||||
(For complete reference list see EL-GPUP Certificate of Analysis) |