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Bet v 1 ELISA kit 白樺過敏原Bet v 1檢測試劑盒
Bet v 1 ELISA kit 白樺過敏原Bet v 1檢測試劑盒
| ELISA kit for measuring Betula verrucosa allergen, Bet v 1. | ||||
| Antibodies: | 4B10/2E10 | |||
| Sensitivity: | 0.2ng/ml | |||
| Limit of Detection: | 3.1ng/ml | |||
| Assay Performance: | Inquire | |||
| Contents: | Monoclonal antibody 4B10, Bet v 1 Standard, Biotinylated antibody 2E10; sufficient reagents for 10 plates. | |||
| Storage: | Store at 4°C. | |||
| Not Provided: | 50mM Carbonate/Bicarbonate buffer, pH 9.6 | |||
| Notes: | ||||
| Product Resources: | ||||
| For research and commercial use in vitro: not for human in vivo or therapeutic use. | ||||
| Certificate of Analysis | ||||
| Monoclonal Antibody: | 4B10 (clone 4B10) | |||
| Vial: | Red Cap; 100μl | |||
| Immunogen: | Bet v 1 | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to multiple isoforms of Betula verrucosa allergen, Bet v 1. | |||
| Purification: | Protein A affinity chromatography | |||
| Concentration: | 1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Monoclonal Antibody: | 2E10 (clone 2E10 G6 G7) | |||
| Vial: | Brown Cap; 100μl | |||
| Immunogen: | Bet v 1 | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to a specific epitope on Betula verrucosa allergen, Bet v 1. | |||
| Purification: | Enrichment and concentration using 50% saturated ammonium sulphate precipitation followed by purification using protein A affinity chromatography. | |||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Allergen Standard: | rBet v 1a | |||
| Vial: | White Cap; 400μl | |||
| Composition: | Recombinant Bet v 1a prepared in 1% BSA/50 glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4. | |||
| Concentration: | 1000ng/ml Bet v 1 | |||
| Calibration: | The rBet v 1a was produced from Pichia and purified by a multi-step chromatography procedure. Validity of the rBet v 1 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001565), recombinant major allergen rBet v 1 containing 10.25μg of rBet v 1 per vial. | |||
| References: | ||||
1. Breiteneder H, Pettenburger K, Bito A, et al. The gene coding for themajor birch pollen allergen Bet v 1, is highly homologous to a peadisease resistance response gene. EMBO J. 1989;8:1935-8. 2. Hoffmann-Sommergruber K, Susani M, Ferreira F, et al. High-levelexpression and purification of the major birch pollen allergen, Bet v 1.Protein Expr Purif. 1997;9:33-9. 3. Godnic-Cvar J, Susani M, Breiteneder H, et al. Recombinant Bet v 1, themajor birch pollen allergen, induces hypersensitivity reactions equal tothose induced by natural Bet v 1 in the airways of patients allergic to treepollen. J Allergy Clin Immu nol. 1997;99:354-9. 4. Schappi GF, Monn C, Wuthrich B, Wanner HU. Direct determination ofallergens in ambient aerosols: methodological aspects. Int Arch AllergyImmunol. 1996;110:364-70. 5. Schappi GF, Suphioglu C, Taylor PE, Knox RB. Concentrations of themajor birch tree allergen Bet v 1 in pollen and respirable fine particles inthe atmosphere. J Allergy Clin Immunol. 1997;100:656-61. 6. S. Vieths, D. Barber, M. Chapman, et al. Establishment of recombinantmajor allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standardsand validation of ELISA methods for their measurement. Pharmeuropa Bio&SN October 2012;118-13. | ||||
| (For complete reference list see EL-BV1 Certificate of Analysis) | ||||
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