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Der p 1 ELISA kit 屋塵螨過敏原Der p 1 檢測試劑盒
Der p 1 ELISA kit 屋塵螨過敏原Der p 1 檢測試劑盒
| ELISA kit for measuring D. pteronyssinus allergen, Der p 1. | ||||
| Antibodies: | 10B9/5H8 | |||
| Sensitivity: | 0.5ng/ml | |||
| Limit of Detection: | 2ng/ml | |||
| Assay Performance: | Inquire | |||
| Contents: | Monoclonal antibody 10B9, Universal Allergen Standard, Biotinylated antibody 5H8; | |||
| Storage: | Store at 4°C. | |||
| Not Provided: | 50mM Carbonate/Bicarbonate buffer, pH 9.6 | |||
| Notes: | ||||
| Product Resources: | EL-DP1A Certificate of Analysis | |||
For research and commercial use in vitro: not for human in vivo or therapeutic use. | ||||
| Certificate of Analysis | ||||
| Monoclonal Antibody: | 10B9 (clone 10B9 F6 A12) | |||
| Vial: | Red Cap; 100μl | |||
| Immunogen: | Der p 1 | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to an epitope on dust mite Dermatophagoides pteronyssinus allergen, Der p 1. | |||
| Purification: | Produced in ascites and purified by ammonium sulphate precipitation and affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
| Concentration: | 1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Monoclonal Antibody: | 5H8 (clone 5H8 C12 D8) | |||
| Vial: | Brown Cap; 100μl | |||
| Immunogen: | Der p 1 | |||
| Isotype: | Mouse IgG2A | |||
| Specificity: | Binds to an epitope on dust mite Dermatophagoides pteronyssinus allergen, Der p 1. | |||
| Purification: | Produced in cell culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Allergen Standard: | Universal Allergen Standard | |||
| Vial: | White Cap; 400μl | |||
| Composition: | A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4 | |||
| Concentration/Calibration: | ||||
| Universal Allergen Standard | Protein Measurement | Concentration (ng/ml) | ||
| Der p 1 | Amino-acid analysis | 2500 | ||
| Der f 1 | Amino-acid analysis | 2500 | ||
| Der p 2 | Amino-acid analysis | 1000 | ||
| Fel d 1 | Amino-acid analysis | 1000 | ||
| Can f 1 | Amino-acid analysis | 2500 | ||
| Rat n 1 | Amino-acid analysis | 1000 | ||
| Mus m 1 | Amino-acid analysis | 250 | ||
| Bla g 2 | Amino-acid analysis | 2500 | ||
References: 1. Luczynska CM, Arruda LK, Platts-Mills TA, Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I. J Immunol Methods 1989; 118(2):227-235. 2. Custovic A, Taggart SC, Francis HC, Chapman MD, Woodcock A. Exposure to house dust mite allergens and the clinical activity of asthma. J Allergy Clin Immunol 1996; 98(1):64-72. 3. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24. 4. Sawyer G, Kemp T, Shaw R et al. Biologic pollution in infant bedding in New Zealand: high allergen exposure during a vulnerable period. J Allergy Clin Immunol 1998; 102(5):765-770. 5. Arbes SJ, Jr., Cohn RD, Yin M et al. House dust mite allergen in US beds: results from the First National Survey of Lead and Allergens in Housing. J Allergy Clin Immunol 2003; 111(2):408-414. 6. Gruchalla RS, Pongracic J, Plaut M et al. Inner City Asthma Study: relationships among sensitivity, allergen exposure, and asthma morbidity. J Allergy Clin Immunol 2005; 115(3):478-485. 7. Zock JP, Heinrich J, Jarvis D et al. Distribution and determinants of house dust mite allergens in Europe: the European Community Respiratory Health Survey II. J Allergy Clin Immunol 2006; 118(3):682-690. 8. Jarvis D, Zock JP, Heinrich J et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol 2007; 119:697-704. 9. Earle CD, King EM, Tsay A et al. High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol 2007; 119(2):428-433. (For complete reference list see EL-DP1 Certificate of Analysis) | ||||
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